Anti-phospho-PKD2 (pSer876) antibody produced in rabbit
SIGMA/SAB4504104 - affinity isolated antibody
Synonym: Anti-HSPC187
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-PKD2 (pSer876): Cat. No. SAB4504104: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-PKD2 (pSer876): Cat. No. SAB4504104: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Western Blotting Anti-phoshpho-PKD2 (pSer876): Cat. No. SAB4504104: Western blot analysis of extracts from NIH-3T3 cells treated with PMA 250ng/mL for 5 minutes.
ELISA Anti-phoshpho-PKD2 (pSer876): Cat. No. SAB4504104: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 96 kDa |
| NCBI accession no. | Q9BZL6   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer876) |
| technique(s) | ELISA: 1:60000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9BZL6 |
| Biochem/physiol Actions: | Protein kinase D2 (PRKD2) is a novel, essential mediator of tumor cell-endothelial cell communication and a promising therapeutic target to inhibit angiogenesis in gastrointestinal cancers. Mutation in the gene leads to autosomal dominant polycystic kidney disease (ADPKD). Polycystin-2 might function as a component of intracellular channel complexes. PRKD2 influences CD8+ T cell-dependent immune responses. PKD2 facilitates cell migration and it is associated with the progression and maintenance of various malignancies. Increased expression of PKD2 is observed in various types of cancers including colorectal cancer, glioblastoma multiforme (GBM). CRT0066101 acts as an inhibitor for all the three protein kinase D isoforms. Therefore, it can be used as a therapeutic drug for PRKD2 induced carcinogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The protein kinase D2 (PRKD2) gene, mapped to human chromosome 4q21-23, codes for integral membrane protein polycystin-2. PRKD2 is a member of the serine/threonine protein kinase D (PRKD) family. It is characterized with two N-terminal cysteine-rich zinc finger domains involved in binding diacylglycerol (DAG) and phorbol esters, a central lipid/protein binding pleckstrin homology (PH) domain, and a C-terminal kinase domain. PRKD2 is mainly expressed in the endoplasmic reticulum (ER). |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser876. Immunogen Range: 829-878 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |