Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit
SIGMA/SAB4504169 - affinity isolated antibody
Synonym: Anti-DNA-PKC; Anti-DNA-PKcs; Anti-DNAPK; Anti-DNAPKc; Anti-DNPK1; Anti-HYRC; Anti-HYRC1; Anti-IMD26; Anti-XRCC7
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Immunohistochemistry Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
ELISA Anti-phoshpho-DNA-PK (pSer2056): Cat. No. SAB4504169: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 469 kDa |
| NCBI accession no. | P78527   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer2056) |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P78527 |
| Application: | Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit has been used in immunofluorescence analysis. |
| Biochem/physiol Actions: | Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, plays an essential role in the colorectal cancer (CRC) cell proliferation/survival. Therefore, it can be used as a potential prognostic biomarker and a therapeutic target for CRC. Decreased expression of PRKDC influences apoptosis partially by inhibiting AKT activation. Overexpression of activated DNAPK elevates the expression of rearranged during transfection (RET), which is a proto-oncogene involved in the development of medullary thyroid cancer (MTC). DNAPK plays a crucial role in non-homology end joining (NHEJ) pathway. Downregulation of DNAPK enhances anticancer drug induced DNA damage repair in osteosarcoma cell line MG63. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, is encoded by the gene mapped to human chromosome 8q11. PRKDC is characterized by a large catalytic polypeptide and DNA-binding component comprised of the Ku70 and Ku80 heterodimer, that targets DNA-PKc, to DNA. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DNA-PK around the phosphorylation site of Ser2056. Immunogen Range: 2022-2071 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |