Anti-phospho-ERK8 (pThr175+Tyr177) antibody produced in rabbit
SIGMA/SAB4504176 - affinity isolated antibody
Synonym: ERK7; MAPK15
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Western Blotting Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: Western blot analysis of extracts from Jurkat cells.
ELISA Anti-phoshpho-ERK8 (pThr175)+Tyr177): Cat. No. SAB4504176: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 59 kDa |
| NCBI accession no. | Q8TD08   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr175+pTyr177) |
| technique(s) | ELISA: 1:5000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q8TD08 |
| Biochem/physiol Actions: | Extracellular signal-regulated kinase 8 (ERK8)/mitogen-activated protein (MAP) kinase 15 aids in maintaining the integrity of the genome. Anaplastic thyroid carcinoma cells show high expression of MAPK15. It controls telomerase reverse transcriptase (hTERT) mRNA expression to regulate telomerase activity. It is highly essential to control the generation of reactive oxygen species. MAPK15 also plays a role in stabilizing proliferating cell nuclear antigen protein. MAPK15 might be involved in the progression of human cancer. Hence it might act as a candidate as an attractive target for cancer therapy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Mitogen-activated protein (MAP) kinase 15 (MAPK15) or extracellular signal-regulated kinase 8 (ERK8) belongs to the MAP kinase family. MAPK15 gene is located on human chromosome 8q24.3. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. Immunogen Range: 141-190 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |