Anti-phospho-Smad2/3 (pThr8) antibody produced in rabbit
SIGMA/SAB4504208 - affinity isolated antibody
Synonym: Anti-HSPC193; Anti-HsT17436; Anti-JV15-2; Anti-LDS1C; Anti-LDS3; Anti-MADH3
Product Type: Chemical
Western Blotting Anti-phoshpho-Smad2/3 (pThr8): Cat. No. SAB4504208: Western blot analysis of extracts from RAW264.7 cells.
ELISA Anti-phoshpho-Smad2/3 (pThr8): Cat. No. SAB4504208: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| NCBI accession no. | P84022   |
| Q15796 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr8) |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P84022 |
| Application: | Anti-phospho-Smad3 (pSer425) antibody has been used in western blotting. |
| Biochem/physiol Actions: | SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein) proteins play an important role in the intracellular signalling of transforming growth factor β (TGFβ). Reduction in the phosphorylation level of Smad3 helps autophagy to control the endothelial-mesenchymal transition. In human arterial smooth muscle cells, suppressing siRNA of SMAD3 improves the viability of cells. Mutations in SMAD2 (SMAD family member 2) results in arterial aneurysms and dissections. Upregulation of Smad2 blocks the multiplication of gingival epithelial cells. It plays an important role in TGF-β (transforming growth factor β)/activin signaling pathways. Smad2 positively and negatively controls TGFβ-dependent transcription with the help of forkhead DNA-binding protein FAST2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | SMAD3 (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein) is located on human chromosome 15q22. SMAD2 (SMAD family member 2) is also called as MADR2 (substrate of the TGFβ receptor). It is located on human chromosome 18q21. Smad2/3 belongs to the mothers against Dpp (MAD) related family of proteins. SMAD2 has two highly conserved amino and carboxyl-terminal domains (MH1 and MH2 domains) but has no known structural motifs. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |