Anti-phospho-Smad3 (pSer204) antibody produced in rabbit
SIGMA/SAB4504210 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human Non-Hodgkin′s lymphoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
Immunohistochemistry Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Western Blotting Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: Western blot analysis of extracts from NIH-3T3 cells treated with 20% serum for 5 minutes.
ELISA Anti-phoshpho-Smad3 (pSer204): Cat. No. SAB4504210: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| NCBI accession no. | P84022   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer204) |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P84022 |
| Application: | Anti-phospho-Smad3 (pSer204) antibody produced in rabbit has been used in western blot analysis.(7) |
| Biochem/physiol Actions: | Smad3 (mothers against decapentaplegic homolog 3) protein identifies tandem repeats of the palindromic sequence GTCTAGAC, which is part of TGF-β (transforming growth factor β) signaling promoter region. Smad3 is associated with TGF-β (transforming growth factor beta) signaling pathway, a cellular process regulating the homeostasis, development, and repair of cartilage. It is known to induce extracellular matrix production. Smad proteins are responsible for the transduction of signals from cell surface to the nucleus. Phosphorylation of Smad3 controls tumor progression, inflammation, fibrosis, obesity and diabetes. Smad3 expression might be associated with the pathogenesis of osteoarthritis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The SMAD3 (mothers against decapentaplegic homolog 3) gene is mapped to human chromosome 15q22.33. SMAD proteins contains three main domains : MH1 (MAD homology 1) - amino terminal domain (highly conserved), MH2 - carboxy terminal domain and a linker domain between the two domains. The MH1 domain of Smad3 serves as a DNA binding motif.(8) |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. Immunogen Range: 170-219 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |