Anti-phospho-p47 phox (pSer359) antibody produced in rabbit
SIGMA/SAB4504289 - affinity isolated antibody
Synonym: Anti-CGD1; Anti-NCF1A; Anti-NOXO2; Anti-SH3PXD1A; Anti-p47phox
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-p47 phox (pSer359): Cat. No. SAB4504289: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Immunohistochemistry Anti-phoshpho-p47 phox (pSer359): Cat. No. SAB4504289: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Western Blotting Anti-phoshpho-p47 phox (pSer359): Cat. No. SAB4504289: Western blot analysis of extracts from HeLa cells treated with nocodazole 1μg/mL 18h.
ELISA Anti-phoshpho-p47 phox (pSer359): Cat. No. SAB4504289: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 44 kDa |
| NCBI accession no. | P14598   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer359) |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P14598 |
| Biochem/physiol Actions: | NCF1 (neutrophil cytosolic factor-1) is an important constituent of the phagocyte NADPH-oxidase system. The enzyme helps in the synthesis of microbicidal superoxide in phagocytes like neutrophil and plays a key role in host defense against microbial pathogens. A 2-bp GT deletion in exon 2 of the NCF1 gene results in chronic granulomatous disease (CGD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Phospho-p47/ NCF1 (neutrophil cytosolic factor-1) codes for the p47phox subunit of the phagocyte NADPH oxidase (NOX2). It is a cytosolic factor. This gene is located with two pseudogenes (NCF1B and NCF1C). NCF1 is mapped to human chromosome 7q11.23. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359. Immunogen Range: 331-380 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |