Anti-phospho-Akt (pThr308) antibody produced in rabbit
SIGMA/SAB4504332 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-Akt (pThr308): Cat. No. SAB4504332: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-Akt (pThr308): Cat. No. SAB4504332: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-Akt (pThr308): Cat. No. SAB4504332: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Western Blotting Anti-phoshpho-Akt (pThr308): Cat. No. SAB4504332: Western blot analysis of extracts from HEK 293 cells treated with Insulin.
ELISA Anti-phoshpho-Akt (pThr308): Cat. No. SAB4504332: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 55 kDa |
| NCBI accession no. | P31749   |
| P31751 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr308) |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P31751 |
| Application: | Anti-phospho-Akt (pThr308) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | AKT is activated in a multistep process that involves its translocation across the membrane and its phosphorylation. The 3′-phosphorylated phosphoinositides, 3,4,5-trisphosphate (PI-3,4,5-P3) and PI-3,4,-P2 produced by PI3K bind to the PH (pleckstrin homology) domain and this binding facilitates the localization of AKT kinases to the plasma membrane. Once localized, PDK1 (3-phosphoinositide-depen |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The AKT/protein kinase B gene has three homologues, namely PKBα (Akt1), PKBβ (Akt2), and PKBγ (Akt3), which are localized to human chromosome 14q32, 19q13, and 1q44, respectively. The members of AKT family contain an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker, and a carboxyl-terminal kinase domain. The AKT1 and AKT2 genes are expressed highly in insulin-responsive tissues, such as brown fat. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Thr308. Immunogen Range: 276-325 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |