Anti-phospho-ASK1 (pSer966) antibody produced in rabbit
SIGMA/SAB4504337 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-ASK1 (pSer966): Cat. No. SAB4504337: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-ASK1 (pSer966): Cat. No. SAB4504337: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Western Blotting Anti-phoshpho-ASK1 (pSer966): Cat. No. SAB4504337: Western blot analysis of extracts from HEK 293 cells treated with TNF(20ng/mL)+calyculinA(50nM) for 5 minutes.
ELISA Anti-phoshpho-ASK1 (pSer966): Cat. No. SAB4504337: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 154 kDa |
| NCBI accession no. | Q99683   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer966) |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q99683 |
| Application: | Anti-phospho-ASK1 (pSer966) antibody has been used in western blotting. |
| Application: | Anti-phospho-ASK1 (pSer966) antibody produced in rabbit has been used in immunoblot analysis. |
| Biochem/physiol Actions: | Apoptosis signal-regulating kinase 1 (ASK1) is linked with apoptosis in cancer and heart tissues. It act as an upstream regulator of p38 and JNK (c-Jun N-terminal kinase) in cardiomyocytes death. Mutations in MAP3K5 gene result in β-thalassemia disease severity and hydroxyurea treatment efficacy. |
| Biochem/physiol Actions: | Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), is one of the vital genes involved in the mitogen-activated protein kinase (MAPK) signaling cascade, which plays a crucial role in the cytoplasmic transduction of extracellular signals. Apoptosis signal-regulating kinase 1 (ASK1)-dependent mitochondrial apoptosis pathway, activated by 4SC-202, which is a novel class I HDAC inhibitor (HDACi), potentially stops hepatocellular carcinoma (HCC) cell proliferation. The encoded protein in response to oxidative stress, endoplasmic reticulum stress, infection and calcium influx plays a vital role in intracellular signaling pathways and biological functions including cell death, differentiation and inflammation. Genomic variation in MAP3K5 indicates β-thalassemia disease severity and hydroxyurea (HU) treatment efficiency. ASK1 plays a vital role in inhibition of the adipocytic differentiation process of the tumor cells and functions as an efficient therapeutic target for several human diseases including malignant fibrous histiocytomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Apoptosis signal-regulating kinase 1 (ASK1) is a serine/threonine protein kinase. It belongs to the MAPKKK (mitogen-activated protein kinase) family. This gene is located on human chromosome 6q23. |
| General description: | Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), is encoded by the gene mapped to human chromosome 6q22.33. The encoded protein belongs to the mitogen-activated protein kinase family. Porcine MAP3K5, which shares 97 % sequence identity with human MAP3K5, is characterized with a domain of unknown function (DUF4071) and a protein kinase domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966. Immunogen Range: 932-981 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |