Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit
SIGMA/SAB4504372 - affinity isolated antibody
Product Type: Chemical
Western Blotting Anti-phoshpho-Cortactin (pTyr421): Cat. No. SAB4504372: Western blot analysis of extracts from HeLa cells treated with H2O2.
ELISA Anti-phoshpho-Cortactin (pTyr421): Cat. No. SAB4504372: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 61 kDa |
| NCBI accession no. | Q14247   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr421) |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q14247 |
| Application: | Anti-phospho-Cortactin (pTyr421) antibody has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | Cortactin controls Arp2/3-mediated actin polymerization. This protein also modulates aggressive cancer cell traits like tumor growth, cancer cell invasion and motility, and serum- and anchorage-independent growth. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Cortactin is an actin-related protein 2/3 (Arp2/3) complex–activating and filamentous (F)-actin–binding protein. This protein is coded by CTTN gene that maps to human chromosome 11q13. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Cortactin around the phosphorylation site of Tyr421. Immunogen Range: 388-437 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |