Anti-phospho-Histone H3.1 (pSer10) antibody produced in rabbit
SIGMA/SAB4504429 - affinity isolated antibody
Synonym: Anti-H3.1; Anti-H3/c; Anti-H3C1; Anti-H3C10; Anti-H3C11; Anti-H3C12; Anti-H3C2; Anti-H3C4; Anti-H3C6; Anti-H3C8; Anti-H3FC; Anti-HIST1H3C
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
Immunohistochemistry Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Immunohistochemistry Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Western Blotting Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: Western blot analysis of extracts from NIH/3T3 cells and HepG2 cells.
ELISA Anti-phoshpho-Histone H3.1 (pSer10): Cat. No. SAB4504429: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 15 kDa |
| NCBI accession no. | P68431   |
| P84243 |
|
| Q71DI3 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer10) |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P68431 |
| Biochem/physiol Actions: | Histone proteins are basic building blocks of chromatin. Mutations in histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that drive chromatin condensation. H3.1 acts as a replication-dependent histone. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). H3.1 is also called as HIST1H3E. It is mainly expressed in the S phase. HIST1H3E is located on human chromosome 6p22. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |