Anti-phospho-mTOR (pSer2448) antibody produced in rabbit
SIGMA/SAB4504476 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Immunohistochemistry Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Western Blotting Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: Western blot analysis of extracts from HeLa cells treated with 200 ng/mL EGF for 30 minutes.
ELISA Anti-phoshpho-mTOR (pSer2448): Cat. No. SAB4504476: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 288 kDa |
| NCBI accession no. | P42345   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer2448) |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P42345 |
| Application: | Anti-phospho-mTOR (pSer2448) antibody has been used in western blotting. |
| Biochem/physiol Actions: | The mechanistic/mammalian target of rapamycin (mTOR) controls protein translation, cell growth, survival, invasion and metabolism. It acts as an intracellular signalling regulator, which links the extracellular events to the intracellular process. mTOR also acts on the protein synthesis machinery. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The mechanistic/mammalian target of rapamycin (mTOR) is a serine/threonine kinase. It is a member of the phosphatidylinositide 3-kinases (PI 3-kinases or PI3Ks) family. It is mapped to human chromosome 1p36.22. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448. Immunogen Range: 2415-2464 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |