Anti-phospho-Src (pTyr418) antibody produced in rabbit
SIGMA/SAB4504536 - affinity isolated antibody
Synonym: Anti-ASV; Anti-SRC1; Anti-THC6; Anti-c-SRC; Anti-p60-Src
Product Type: Chemical
Western Blotting Anti-phoshpho-Src (pTyr418): Cat. No. SAB4504536: Western blot analysis of extracts from COLO205 cells treated with 15% serum for 10 minutes.
ELISA Anti-phoshpho-Src (pTyr418): Cat. No. SAB4504536: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 59 kDa |
| NCBI accession no. | P12931   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr418) |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P12931 |
| Application: | Anti-phospho-Src (pTyr418) antibody has been used in immunofluorescence. |
| Biochem/physiol Actions: | SRC (proto-oncogene, non-receptor tyrosine kinase) controls early HIV-1 (human immunodeficiency virus 1) infection post-entry. This gene participates in fundamental physiological and pathological processes like carcinogenesis and tumor progression. Src kinase activity helps in the migration of endothelial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | SRC (proto-oncogene, non-receptor tyrosine kinase) belongs to the Src family kinases (SFKs). It contains an amino-terminal domain, conserved Src-homology domains (SH2 and SH3), a tyrosine kinase domain and the carboxyl-terminus. This gene is located on human chromosome 20q11. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr418. Immunogen Range: 386-435 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |