Anti-phospho-p47 phox (pSer345) antibody produced in rabbit
SIGMA/SAB4504721 - affinity isolated antibody
Synonym: Anti-CGD1; Anti-NCF1A; Anti-NOXO2; Anti-SH3PXD1A; Anti-p47phox
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Western Blotting Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: Western blot analysis of extracts from HepG2 cells treated with 20 ng/mL TNF-α for 5 minutes.
ELISA Anti-phoshpho-p47 phox (pSer345): Cat. No. SAB4504721: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 44 kDa |
| NCBI accession no. | P14598   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer345) |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P14598 |
| Biochem/physiol Actions: | Neutrophil cytosolic factor 1 (NCF1) stimulates the production of reactive oxygen species (ROS) under hyperoxic condition. The above process can be inhibited by the NCF1 inhibitor diphenylene iodonium (DPI) and apocynin. Mutation in the gene leads to a rare congenital immune deficiency called, chronic granulomatous disease (CGD). Deletion of NCF1 gene, which is a vital component of NADPH oxidase complex implicated in generation of oxidative stress, leads to Williams syndrome. It also act as a modifier of vascular stiffness. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The neutrophil cytosolic factor 1 (NCF1), also known as p47phox gene, spanning 15kb with 11 exons, is mapped to human chromosome 7q11.23. The gene codes for a 390 amino acid protein, which is a novel component of the NADPH oxidase complex. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser345. Immunogen Range: 311-360 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |