Anti-Rabbit IgG (H+L), CF™ 488A antibody produced in chicken
SIGMA/SAB4600030 - ~2 mg/mL, affinity isolated antibody
Product Type: Chemical
Immunocytochemistry Immunofluorescence staining of HeLa cells with an antibody against a Golgi marker detected using a CF405S, blue, secondary antibody conjugate and Anti-Tubulin antibody detected using a CF488A, green, secondary antibody conjugate. Actin filaments are stained with CF555, red, phalloidin conjugate.
Chart Absorption and emission spectra of CF488A conjugated to Goat Anti-Mouse IgG in 1× PBS buffer.
Immunohistochemistry Frozen section of Rat optic nerve stained with Mouse Anti-neurofilament H and CF568 Goat Anti-Mouse (neuronal processes, red), Rabbit Anti-GFAP and CF488A Goat Anti-Rabbit, highly cross-adsorbed (glial cells, green). Nuclei are stained with RedDot2 (cyan).
Immunocytochemistry HeLa cells stained with Mouse Anti-tubulin and CF488A Goat Anti-Mouse (microtubules, green). Actin filaments are stained with CF640R phalloidin (magenta). Nuclei are counterstained with DAPI (blue).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | chicken |
| clone | polyclonal |
| concentration | ~2 mg/mL |
| conjugate | CF™ 488A conjugate |
| fluorescence | λex 490 nm; λem 515 nm |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1-10 μg/mL |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect immunofluorescence: 1-10 μg/mL |
| Application: | Anti-Rabbit IgG (H+L), CF™ 488A antibody has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Binds all rabbit IgGs |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Immunogen: | rabbit IgG (H+L) |
| Legal Information: | CF is a trademark of Biotium, Inc. |
| Legal Information: | This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer’s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com. |
| Physical form: | Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin. |
| Preparation Note: | Protect from light. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |