Anti-Rat IgG (H+L), highly cross-adsorbed, CF™ 640R antibody produced in donkey
SIGMA/SAB4600156 - ~2 mg/mL, affinity isolated antibody
Product Type: Chemical
Chart Absorption and emission spectra of CF640R conjugated to Goat Anti-Mouse IgG in 1× PBS buffer.
Immunocytochemistry Immunofluorescence staining of HeLa cells with Anti-Tubulin antibody detected using a CF640R, red, secondary antibody conjugate.
Immunocytochemistry Relative photostability of CF640R and AlexaFluor 647. HeLa cells were stained with Anti-beta-tubulin antibody conjugates of CF640R or AlexaFluor 647. Cells were continuously illuminated by a mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera . Images were captured at 0 minutes, 1 minute, and 3 minutes.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | donkey |
| clone | polyclonal |
| concentration | ~2 mg/mL |
| conjugate | CF™ 640R conjugate |
| fluorescence | λex 642 nm; λem 662 nm |
| form | buffered aqueous solution |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1-10 μg/mL |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect immunofluorescence: 1-10 μg/mL |
| Application: | Anti-Rat IgG (H+L), highly cross-adsorbed, CF™ 640R antibody produced in donkey has been used in whole mount staining for NG2+ pericytes. |
| Biochem/physiol Actions: | Binds all rat IgGs (minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep serum proteins) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.  |
| General description: | Immunoglobulin G (IgG) is a major class of immunoglobulin family and is a largely expressed serum antibody. IgG consists of gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1,γ2, γ3, and γ4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. |
| Immunogen: | rat IgG (H+L) |
| Legal Information: | CF is a trademark of Biotium, Inc. |
| Legal Information: | This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer’s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com. |
| Physical form: | Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin. |
| Preparation Note: | Protect from light. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |