Monoclonal Anti-CD3-FITC antibody produced in mouse
SIGMA/SAB4700048 - clone MEM-57, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Flow cytometry Monoclonal Anti-CD3-FITC: Cat. No. SAB4700048: Flow cytometry analysis of Human peripheral blood cells with Anti-Human CD3 FITC (lymphocyte gate).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MEM-57, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| NCBI accession no. | NM_000733.3   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| Application: | The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests. |
| Biochem/physiol Actions: | T-cell receptor-CD3 complex containing CD3ε, CD3γ and CD3δ, is essentially involved in inducing early metabolic events that lead to T-cell activation. Mutation in the CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CD3 molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes. |
| General description: | The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. |
| Immunogen: | Human thymocytes and T lymphocytes |
| Physical form: | Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |