Monoclonal Anti-CD140a-PE antibody produced in mouse
SIGMA/SAB4700457 - clone 16A1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PDGF-RA; Anti-PDGFRA
Product Type: Chemical
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Anti-human CD140a PE antibody (clone 16A1) specificity verification by flow cytometry
Anti-human CD140a (16A1) PE antibody (concentration in sample 6 μg/ml, red-filled histogram) binds specifically to CD140a expressed on surface of PDGFRA transfected 3T3 cells (upper panel), but not to surface of non-transfected 3T3 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 6 μg/ml, black-dashed histogram).
Anti-human CD140a (16A1) PE antibody (concentration in sample 6 μg/ml, red-filled histogram) binds specifically to CD140a expressed on surface of PDGFRA transfected 3T3 cells (upper panel), but not to surface of non-transfected 3T3 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 6 μg/ml, black-dashed histogram).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 16A1, monoclonal |
| conjugate | phycoerythrin (R-PE) conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| NCBI accession no. | NM_006206.4   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| UniProt accession no. | P16234 |
| Application: | The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours. |
| Immunogen: | CD140a-transfected NIH 3T3 cells |
| Physical form: | Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |