Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse
SIGMA/SAB4700630 - clone MoBu-1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BrdU
Product Type: Chemical
Flow cytometry Monoclonal Anti-5-bromodeoxyuridine: Cat. No. SAB4700630: Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM Human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat Anti-Mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MoBu-1, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| Application: | Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse has been used in Immunocytochemistry. The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. |
| Biochem/physiol Actions: | 5-Bromodeoxyuridine (BrdU) is an analogue of thymidine, used to measure DNA synthesis immunochemically in living cells. BrdU along with cAMP and butyrate plays a vital role in the cellular differentiation in several mammalian cell types. It also inhibits cell growth in mammalian and yeast cells by modulating the expression of particular genes involved in cell division. In the absence of DNA synthesis, brdu promotes mouse neuroblastoma C1300 cell differentiation into cells that morphologically are similar to mature neurons. BrdU activates cell senescence-like phenomenon in mammalian cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). |
| Immunogen: | 5-bromodeoxyuridine conjugated with hemocyanine |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |