Monoclonal Anti-HLA Class I, low endotoxin antibody produced in mouse
SIGMA/SAB4700637 - clone W6/32, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
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| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | W6/32, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| impurities | ≤1 EU/μg endotoxin (LAL test) |
| isotype | IgG2a |
| NCBI accession no. | NM_002116.6   |
| NM_002117.4 |
|
| NM_005514.6 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| UniProt accession no. | P04439 |
| Application: | The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 2-8 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. |
| Biochem/physiol Actions: | Human leukocyte antigen A class I downregulation is implicated in tumor invasion and development. Genetic variations in the class I major histocompatibility complex (MHC) is associated with the development of melanoma. HLA-viral peptide interaction plays a major role in the regulation of human immunodeficiency virus (HIV) infection. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Human leukocyte antigen A classical class I family consists of three members including HLA-A, HLA-B and HLA-C. Members of classical class I major histocompatibility complex (MHC) contain a peptide bound to a transmembrane glycoprotein subunit, the heavy chain, in association with a soluble light chain called β2-microglobulin. Class I MHC molecules are present in changeable volumes on all nucleated cells. |
| General description: | The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database. |
| Immunogen: | Membrane of human tonsil cells |
| Physical form: | Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |