Monoclonal Anti-DNA/RNA Damage antibody produced in mouse
SIGMA/SAB5200010 - clone 15A3, 1 mg/mL, purified immunoglobulin
Synonym: Anti-8 hydroxy guanine; Anti-8-OH-dG; Anti-8OHG; Anti-DNA/RNA Damage
Product Type: Chemical
Immunofluorescence Monoclonal Anti-DNA/RNA Damage, Clone 15A3: Cat. No. SAB5200010: Immunofluorescence of oxidized 8-OH-dG in ischemic rat brain tissue. Left to right: 8-OH-dG, DAPI, merged.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 15A3, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG2b |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1000 |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable |
| Biochem/physiol Actions: | 8-hydroxyguanine (8-OHG) helps in determining the reactive oxygen species (ROS)-induced modification of a purine residue in DNA. It is a sensitive marker of oxidative DNA damage. Overexpression of the gene has been observed in diabetic patients, especially those with advanced microvascular complications. Thus, 8-OHG functions as a novel biomarker for identifying oxidative DNA damage in diabetic patients. Plasma level of 8-OHG elevates with the age, with cigarette smoking and during tumorigenesis. 8-OHG levels in urine is considered as a biomarker of the total systemic oxidative stress in vivo. Overexpression of 8-OHG has been observed in various neurodegenerative diseases such as Parkinson’s disease (PD) and multiple system atrophy (MSA). |
| Biochem/physiol Actions: | DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage. 8-hydroxyguanine, 8-hydroxy-2’-eoxyguanonsi |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | DNA/RNA damage, also known as 8-hydroxyguanine (8-OHG), is a product of oxidized DNA or RNA. |
| Immunogen: | 8-hydroxy-guanosine-BSA and casein conjugates |
| Physical form: | Solution in PBS, 50% glycerol, and 0.09% sodium azide |
| Specificity: | Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanos |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |