Monoclonal Anti-HIF-1 α antibody produced in mouse
SIGMA/SAB5200017 - clone ESEE122, 1 mg/mL, purified immunoglobulin
Synonym: Anti-ARNT interacting protein; Anti-HIF1 alpha, ESEE122; Anti-HIF1A; Anti-Hypoxia inducible factor 1 alpha; Anti-MOP1; Anti-PASD8
Product Type: Chemical
Immunofluorescence Monoclonal Anti-HIF-1 α, Clone ESEE122: Cat. No. SAB5200017: Immunofluoresce of HIF-1 α on mouse backskin sections.
Immunoblotting Monoclonal Anti-HIF-1 α, Clone ESEE122: Cat. No. SAB5200017: Western Blot analysis of HIF-1 α in HeLa cell lysates, using a 1:500 antibody dilution.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ESEE122, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| NCBI accession no. | NP_034561.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | bovine, mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q16665 |
| Application: | Monoclonal Anti-HIF-1 α antibody produced in mouse has been used in siRNA assay and western blot analysis. |
| Biochem/physiol Actions: | Hypoxia-inducible factor-1(HIF1) plays an essential role in cellular and systemic homeostatic responses to hypoxia. During hypoxia, the two subunits of this factor undergo post-translational modifications which in turn promote transactivation. HIF-1α gene loss is associated with development of nonpapillary renal carcinomas. The encoded protein functions as a key regulator of the vascular endothelial growth factor (VEGF) under hypoxic response in human stromal cells in vitro. HIF-1α protein expression is often seen in invasive breast cancer. Experimental data demonstrates that downregulation of HIF-1α decreases tumorigenicity of MCF-7 (human breast adenocarcinoma cell line) cells and propose a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment. |
| Biochem/physiol Actions: | Specific for HIF1-alpha. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Hypoxia-inducible factor-1 α (HIF1 α) gene encodes the α subunit of HIF-1. HIF-1α contains oxygen-dependent degradation (ODD) domain that mediates the interaction between HIF-1α and the E3 ubiquitin ligase complex, which in turn facilitates continuous poly ubiquitination of HIF-1α in normoxic cells. The gene encoding it is localized on human chromosome 14q23.2. HIF1 α belongs to the basic helix-loop-helix family of transcription factors. |
| Immunogen: | Recombinant fragment corresponding to amino acids 329-530 |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |