Monoclonal Anti-CACNA1D antibody produced in mouse
SIGMA/SAB5200022 - clone S48A-9, 1 mg/mL, purified immunoglobulin
Synonym: Anti-CACH3; Anti-CACN4; Anti-CACNA 1D; Anti-CACNL1A2; Anti-Cav1.3, S48A-9; Anti-oltage dependent L type calcium channel subunit alpha 1D
Product Type: Chemical
Immunohistochemistry Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 . Tissue: backskin. Species: Mouse. Fixation: Bouin′s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 . Tissue: hippocampus. Species: Human. Fixation: Bouin′s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3 Monoclonal Antibody, Clone S48 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3 Antibody (D) Composite.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S48A-9, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG2aκ |
| mol wt | antigen predicted mol wt 250 kDa |
| NCBI accession no. | NP_058994.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q01668 |
| Application: | Monoclonal Anti-CACNA1D antibody produced in mouse has been used in immunocytochemistry. |
| Biochem/physiol Actions: | Calcium channel, L type α-1 polypeptide isoform 2 (CACNA1D) is crucial for the functioning of the brain and in hearing. It gets activated at a fast rate and withstands high inward Ca2+ currents even at threshold voltages. CACNA1D aids in dendritic signals linked to mitochondrial stress in the brain and is important for estrogen-stimulated Ca2+ influx. Studies have shown that in atrial myocytes, the C terminus of the channel translocates to the nucleus and regulates Ca2+-activated K+ channels. CACNA1D was shown to be upregulated in endometrial cancer and its knockdown showed a reduction in cell proliferation in vitro. Mutations in the gene encoding it has been associated with aldosterone-producing adenomas (APAs), where it leads to the formation of small adenomas. |
| Biochem/physiol Actions: | Detects ~250 kDa. No cross-reactivity against Cav1.2 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Calcium channel, L type α-1 polypeptide isoform 2 (CACNA1D) is expressed in many tissues and possesses six transmembrane segments, along with a loop lining the pore of the channel. The gene encoding CACNA1D is localized on human chromosome 3p14.3. |
| Immunogen: | Fusion protein amino acids 859-875 of rat Cav1.3, Accession Number P27732 |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |