Monoclonal Anti-HCN4 antibody produced in mouse
SIGMA/SAB5200035 - clone S114-10, 1 mg/mL, purified immunoglobulin
Synonym: Anti-HCN4, S141-10; Anti-
Product Type: Chemical
Immunohistochemistry Immunohistochemistry analysis using mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 . Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.
Immunohistochemistry Immunohistochemistry analysis using mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 . Tissue: hippocampus. Species: Human. Fixation: Bouin′s Fixative and paraffin-embedded. Primary Antibody: mouse Anti-HCN4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-mouse (green) at 1:50 for 1 hour at RT
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: mouse Anti-HCN4 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.
Western Blotting Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 . Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-mouse IgG: HRP for 1 hour at RT.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S114-10, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 130 kDa |
| NCBI accession no. | NP_067690.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9Y3Q4 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) is one of the subunits of hyperpolarization-activat |
| Immunogen: | Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4, Accession Number Q9JKA7 |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| Specificity: | Detects ~130 kDa. No cross-reactivity against other HCNs |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |