Monoclonal Anti-KCNT1 antibody produced in mouse
SIGMA/SAB5200036 - clone S3-26, 1 mg/mL, purified immunoglobulin
Synonym: Anti-SLACK potassium channel, S3-26; Anti-potassium channel, subfamily T, member 1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry analysis using mouse Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 . Tissue: backskin. Species: mouse. Fixation: Bouin′s Fixative and paraffin-embedded. Primary Antibody: mouse Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using mouse Slo2.2 Monoclonal Antibody, Clone N3/26 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: mouse Slo2.2 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Slo2.2 Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using mouse Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: mouse Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.
Western Blotting Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using mouse Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 . Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: mouse Slo2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-mouse IgG: HRP for 1 hour at RT.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S3-26, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 140 kDa |
| NCBI accession no. | NP_068625   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human (weak), mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q5JUK3 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack); NP_068625 |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| Specificity: | Detects ~140 kDa. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |