Monoclonal Anti-SHANK2 antibody produced in mouse
SIGMA/SAB5200041 - clone S23B-6, 1 mg/mL, purified immunoglobulin
Synonym: Anti-SHANK2, S23b-6
Product Type: Chemical
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 Antibody. (D) Composite.
Western Blotting Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 . Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S23B-6, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 160 kDa |
| NCBI accession no. | NP_958738.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UPX8 |
| Biochem/physiol Actions: | Detects ~160 kDa. No cross-reactivity against Shank1 or Shank3. Mouse with weak species reactivity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (SH3 and multiple ankyrin repeat domains protein 2 (Accession Number Q9QX74) |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |