Monoclonal Anti-GABRB3 antibody produced in mouse
SIGMA/SAB5200049 - clone S87-25, 1 mg/mL, purified immunoglobulin
Synonym: Anti-GABA(A) receptor subunit beta3; Anti-GABAAR Beta3, S87-25; Anti-Gabrb3
Product Type: Chemical
Immunohistochemistry Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 . Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.
Western Blotting Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 . Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S87-25, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 55 kDa |
| NCBI accession no. | NP_032097.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P28472 |
| Biochem/physiol Actions: | γ-aminobutyric acid type A receptor β3 subunit (GABRB3) is a candidate gene for autism spectrum conditions (ASC). Alteration in the gene results in increased tactile sensitivity, or hypersensitivity. Overexpression of GABRB3 might be implicated in the pathogenesis of heroin dependence. Aberration or mutation of this gene leads to neurodevelopmental disorders, such as Angelman syndrome, Prader-Willi syndrome and schizophrenia. GABRB3 polymorphisms results in nonsyndromic cleft lip and/or palate (NSCL/P). |
| Biochem/physiol Actions: | Detects ~55 kDa. No cross-reactivity against GABA-A-R-Beta 2 or –Beta1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The γ-aminobutyric acid type A receptor β3 subunit (GABRB3) gene with nine exons spanning 250kb, is mapped to human chromosome 15q12, a hot region of genomic rearrangements. The gene codes for β3 subunit of GABAA receptor, which belongs to the GABA (γ-aminobutyric acid) receptor gene family. |
| Immunogen: | Fusion protein amino acids 370-433 of mouse GABA-A_r Beta3, Accession Number AAB60502. |
| Physical form: | Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |