Anti-APP antibody produced in rabbit
SIGMA/SAB5200113 - 1 mg/mL, affinity isolated antibody
Synonym: Anti-A11; Anti-Amyloid Oligomer αβ; Anti-Amyloid Oligomers (A11)
Product Type: Chemical
Immunoblotting Anti-APP: Cat. No. SAB5200113: Dot Blot analysis of APP of Aβ42 and polyQ36 prefibrillar oligomers and fibrils. Aβ42 and polyQ fibrils only stain with an Amyloid Fibrils antibody (OC), while Aβ42 and polyQ prefibrillar oligomers only react with Anti-APP antibody, Cat. No. SAB5200113 (A11). Picture courtesy of Kayed et al., (2007). Molecular Neurodegeneration, 2: 18.
Immunoblotting Anti-APP: Cat. No. SAB5200113: Western Blot analysis of APP of Aβ42 fibrils and prefibrillar oligomers. Aβ42 fibrils (F) and prefibrillar oligomers (O) were run on SDS polyacrylamide gels, transferred to nitrocellulose and probed with an Amyloid Fibrils antibody (OC) and Anti-APP antibody, Cat. No. SAB5200113 (A11). Picture courtesy of Kayed et al., (2007). Molecular Neurodegeneration, 2: 18.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| NCBI accession no. | NM_000484.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, eukaryotes, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: suitable | |
| UniProt accession no. | P05067 |
| Application: | Anti-APP antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Amyloid β precursor protein (APP) regulates cell signaling. Mutation in this gene is associated with Alzheimer′s disease. It is implicated in synaptic plasticity, dendritic morphogenesis and neuroprotection. APP modulates synaptic vesicle proteins and synaptic transmission. It is involved in GABAergic transmission and maintains the excitatory/inhibitory (E/I) balance. |
| Biochem/physiol Actions: | Amyloid precursor protein (APP) plays a key role in Wnt signalling. It helps to increase synaptoxicity, that is facilitated by Dickkopf-1 (Dkk1). Mutations on this gene results in autosomal dominant early-onset Alzheimer disease (ADEOAD)/hereditary dementia with cerebral amyloid angiopathy (CAA). |
| Biochem/physiol Actions: | Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Amyloid β precursor protein (APP) is a type 1 transmembrane protein. The gene is located on human chromosome 21q21.3. |
| Immunogen: | Synthetic molecular mimic of soluble oligomers. |
| Physical form: | PBS, 50% glycerol, and 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |