Monoclonal Anti-Trap1 - PerCP antibody produced in mouse
SIGMA/SAB5200129 - clone 3H4-2H6, purified immunoglobulin
Synonym: Anti-Heat shock protein 75; Anti-Hsp75; Anti-Hsp90L; Anti-TNFR associated protein 1
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-Trap1: Western blot of Human and Rat lysates showing cross-species detection of ~75 kDa TRAP1 using TRAP1 antibody, clone 3H4-2H6. Anti-TRAP1 Antibody dilution was 1 μg/mL (1:1,000 dilution). Load: 20 μg lysate per lane. Block: 5% milk + TBST 60 min at RT. Primary antibody: Mouse Anti-TRAP1 Monoclonal antibody, clone 3H4-2H6 incubated for 60 min at RT. Secondary antibody: Goat Anti-Rabbit HRP antibody at 1:2,000 for 60 min at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed size: ~75 kDa. Other band(s): ~None. Lanes 1. MW ladder 2. Human lysate, A431 3. Rat lysate, Rat Brain Membrane (RBM)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H4-2H6, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Peridinin-Chlorophyll-Pro |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen predicted mol wt 75 kDa |
| NCBI accession no. | NP_057376.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q12931 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Purified recombinant TRAP1 |
| Physical form: | PBS pH7.4, 50% glyercol, 0.09% sodium azide |
| Specificity: | Detects Trap1. Detects ~75kDa. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |