Monoclonal Anti-Alginate - Atto 594 antibody produced in mouse
SIGMA/SAB5200136 - clone 4B10-1C5, purified immunoglobulin
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-Alginate: Western blot of BSA: Alginate conjugate showing detection of ~50-<250 kDa BSA: Alginate conjugate using Anti-Alginate, clone 4B10-1C5. Block: 5% milk + TBST 60 min at RT. Primary antibody: Mouse Monoclonal Anti-Alginate, clone 4B10-1C5 1:500/TBS, incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:100 for 60 min at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed size: ~50 -<250kDa. Lanes 1. MW ladder 2. 0. 156 μg BSA: Alginate 3. 0. 313 μg BSA: Alginate 4. 0. 625 μg BSA: Alginate 5. 1. 25 μg BSA: Alginate 6. 2. 50 μg BSA: Alginate 7. 5. 00 μg BSA: Alginate 8. 10. 0 μg BSA: Alginate 9. 10 μg BSA, (negative control)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B10-1C5, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Atto 594 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Sodium Alginate conjugated to KLH |
| Physical form: | PBS pH7.4, 50% glyercol, 0.09% sodium azide |
| Specificity: | Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |