Monoclonal Anti-Grp78 - Atto 390 antibody produced in mouse
SIGMA/SAB5200187 - clone 3G12-1G11, purified immunoglobulin
Synonym: Anti-AL022860; Anti-Endoplasmic reticulum lumenal; Anti-HSPA5; Anti-Heat shock 70 kDa protein 5; Anti-MIF2
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-GRP78: Western blot detection of ~78 kDa GRP78 protein using GRP78 antibody, clone 3G12-1G11. Load: 20 μg lysate per lane. Block: 5% milk + TBST 60 min at RT. Primary antibody: Mouse Anti-GRP78, clone 3G12-1G11, incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody for 60 min at RT (1:50/blocking buffer). Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~78 kDa. Lanes 1. MW ladder 2. Human HEK-293 3. Mouse NIH3T3 4. Rat Brain
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G12-1G11, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Atto 390 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen predicted mol wt 70 kDa |
| NCBI accession no. | NP_037215.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| western blot: suitable | |
| UniProt accession no. | P11021 |
| Biochem/physiol Actions: | Detects a protein with a mass of ~78kDa corresponding to GRP78 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Full-length recombinant rat GRP78 |
| Physical form: | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |