Monoclonal Anti-Mmp9 - Fitc antibody produced in mouse
SIGMA/SAB5200306 - clone S51-82, purified immunoglobulin
Synonym: Anti-82kDa matrix metalloproteinase-9; Anti-CLG4B; Anti-GELB; Anti-MANDP2; Anti-MMP-9
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 . Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 . Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.
Western Blotting Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 . Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S51-82, monoclonal |
| concentration | 1 mg/mL |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen predicted mol wt 92 & 82 kDa |
| NCBI accession no. | NP_112317.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | P14780 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fusion protein amino acids 1-708 (full length) of rat MMP9 |
| Physical form: | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Specificity: | Detecs ~92kDa and ~82kDa (pro and active forms) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |