Monoclonal Anti-Notch1 - Biotin antibody produced in mouse
SIGMA/SAB5200917 - clone S253-32, purified immunoglobulin
Synonym: Anti-Motch; Anti-Neurogenic locus notch homolog protein 1; Anti-Neurogenic locus notch protein homolog; Anti-TAN1
Product Type: Product-on-demand
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.
Western Blotting Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 . Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). . Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S253-32, monoclonal |
| concentration | 1 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt >270, ~120 kDa |
| NCBI accession no. | NP_032740.3   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q07008 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 |
| Physical form: | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |