Monoclonal Anti-Protocadherin Gamma (Pan) antibody produced in mouse
SIGMA/SAB5201338 - clone S159-5, purified immunoglobulin
Synonym: Anti-PCDH
Product Type: Product-on-demand
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cell Projection, Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) FGFA/FHFA (pan) Antibody. (D) Composite.
Western Blotting Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGFA/FHFA (pan) protein using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S159-5, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 100 kDa |
| NCBI accession no. | NP_291062.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids). |
| Physical form: | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |