Monoclonal Anti-Hsf1 - Apc antibody produced in rat
SIGMA/SAB5201708 - clone 10H4, purified immunoglobulin
Synonym: Anti-HSTF1
Product Type: Product-on-demand
Immunocytochemistry Monoclonal Anti-HSF1: Immunocytochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody. Tissue: Heat Shocked HeLa Cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4 °C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 120 min at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100×.
Immunoblotting Monoclonal Anti-HSF1: Western blot of human lysates showing detection of ~85 and 95 kDa HSF1 protein using HSF1 antibody, clone 10H4. Block: 5% milk + TBST 60 min at RT. Primary antibody: Rat Monoclonal Anti-HSF1, clone 10H4 incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1,000) for 60 min at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~85 and 95 kDa. Other band(s): ~110, 140, 245 kDa. Lanes 1. MW ladder 2. A431 lysate, 20 μg 3. HEK293 lysate, 20 μg
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 10H4, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Allophycocyanin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 85 kDa |
| NCBI accession no. | NP_032322.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, mouse, monkey, human, rabbit, hamster, rat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q00613 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Purified recombinant mouse HSF1 protein |
| Physical form: | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |