Monoclonal Anti-Tcp1 Alpha - Atto 390 antibody produced in rat
SIGMA/SAB5201735 - clone 23c, purified immunoglobulin
Synonym: Anti-AI528772; Anti-Ccta; Anti-Tric; Anti-p63
Product Type: Product-on-demand
Immunocytochemistry Monoclonal Anti-TCP1-alpha: Immunocytochemistry analysis using Rat Anti-TCP1-alpha Monoclonal Antibody. Tissue: Heat Shocked HeLa Cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4 °C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 120 min at RT. Localization: Cytoplasm. Centrosome. Magnification: 100×.
Immunoblotting Monoclonal Anti-TCP1-alpha: Western blot of human lysates showing detection of ~60 kDa TCP1-alpha protein using TCP1 alpha antibody, clone 23c. Block: 5% milk + TBST 60 min at RT. Primary antibody: Rat Monoclonal Anti-TCP1-alpha, clone 23c incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1,000) for 60 min at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~60 kDa. Other band(s): ~90 kDa. Lanes 1. MW ladder 2. A431 lysate, 20 μg 3. HEK293 lysate, 20 μg
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 23c, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Atto 390 conjugate |
| form | buffered aqueous solution |
| isotype | IgG2c |
| mol wt | antigen predicted mol wt 60 kDa |
| NCBI accession no. | NP_038714.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, dog, rabbit, rat, hamster, bovine, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q32L40 |
| Biochem/physiol Actions: | Tcp1 (t-complex protein) is involved in protein folding mechanism of essential proteins such as actins, tubulin, cyclin E, α-transducin and von Hippel Lindau protein. Tcp1 participates in synthesis of proteins that make up the cytoskeleton and thus involved in cellular processes such as chromosome segregation, cell migration, and intracellular transport. Increased TCP1 gene expression is observed in cancer and upregulation elevates with the disease progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Tcp1 (t-complex protein ) is a chaperonin and a ATPase complex that encodes a chaperonin, localized to the cytosol. |
| Immunogen: | Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group. |
| Physical form: | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |