Monoclonal Anti-Tcp1 Alpha - Apc antibody produced in rat
SIGMA/SAB5201762 - clone 91a, purified immunoglobulin
Synonym: Anti-AI528772; Anti-Ccta; Anti-Tric; Anti-p63
Product Type: Product-on-demand
Immunocytochemistry Monoclonal Anti-TCP1-alpha: Immunocytochemistry analysis using Rat Anti-TCP1-alpha Monoclonal Antibody. Tissue: Heat Shocked HeLa Cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4 °C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 120 min at RT. Localization: Cytoplasm. Centrosome. Magnification: 100×.
Immunoblotting Monoclonal Anti-TCP1-alpha: Western blot of human lysates showing detection of ~60 kDa TCP1-alpha protein using TCP1 alpha antibody, clone 91a. Block: 5% milk + TBST 60 min at RT. Primary antibody: Rat Monoclonal Anti-TCP1-alpha, clone 91a incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1,000) for 60 min at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~60 kDa. Other band(s): ~None. Lanes 1. MW ladder 2. A431 lysate, 20 μg 3. HEK293 lysate, 20 μg
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 91a, monoclonal |
| concentration | 1 mg/mL |
| conjugate | Allophycocyanin conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen predicted mol wt 60 kDa |
| NCBI accession no. | NP_038714.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, rabbit, bovine, monkey, hamster, rat, human, mouse, Saccharomyces cerevisiae, dog, pig, Caenorhabditis elegans, Drosophila melanogaster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | P17987 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region). |
| Physical form: | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |