Monoclonal Anti-ENaC alpha antibody produced in mouse
SIGMA/SAB5201824 - clone 14E10
Product Type: Chemical
Immunohistochemistry (paraffin) Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.
Western Blotting Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85 kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85 kDa.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 14E10, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 85kDa |
| NCBI accession no. | NP_113736   |
| shipped in | wet ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:100 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68) |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |