Monoclonal Anti-PP5 antibody produced in mouse
SIGMA/SAB5201860 - clone 12F7
Product Type: Chemical
Western Blotting Western Blot of A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using PP5 Antibody, clone 2E11. Lane 1: MW Ladder. Lane 2: A431 (15 μg). Lane 3: HEK293 (15 μg). Lane 4: Jurkat (15 μg). Block: 5% Skim Milk (RT, 60 minutes). Primary Antibody: Mouse Anti-PP5, clone 2E11 at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa. Other Band(s): None.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:50 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: HeLa. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus | Cytoplasm. Magnification: 40X.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 12F7, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 58kDa |
| NCBI accession no. | NP_006238.1   |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:100 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Full length human PP5 protein |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |