Monoclonal Anti-HSP70-Atto 488 antibody produced in mouse
SIGMA/SAB5201880 - clone 1H11
Product Type: Chemical
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis on permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing faint cell membrane and intracellular staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: 0.2% PBST. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, faint intracellular. (A) DAPI nuclear stain. (B) Wheat germ agglutinin red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
FACS Fluorescence-activated cell sorting (FACS) using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody, or (B) Anti-HSP70 antibody, clone 1H11, for 40 min at 4 °C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 °C. Analysis shows that Anti-HSP70 can detect low levels of cell surface HSP70, and can fainlty detect intracellular HSP70. Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
Western Blotting Western Blot analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 μg. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H11, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | Atto 488 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 70 kDa |
| NCBI accession no. | NP_005336.3   |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | flow cytometry: 1:250 |
| immunofluorescence: 1:100 | |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Human native HSP70 protein |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |