Monoclonal Anti-CD74-Biotin antibody produced in mouse
SIGMA/SAB5201979 - clone 6D9
Product Type: Chemical
Western Blotting Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Tissue: HeLa. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Red F-Actin stain (C) CD74 Antibody (D) Composite.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D9, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 33 kDa |
| NCBI accession no. | NP_001020329.1   |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:100 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |