Monoclonal Anti-beta 4 Spectrin-PerCP antibody produced in mouse
SIGMA/SAB5202073 - clone S393-2, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SPTBN4
Product Type: Chemical
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 3 Antibody (D) Composite.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 3 Antibody. (D) Composite.
Western Blotting Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 . Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | monoclonal |
| S393-2 | |
| concentration | 1.0 mg/mL |
| conjugate | Peridinin-Chlorophyll-Pro |
| description | clone S393-2, purified immunoglobulin, buffered aqueous solution |
| target molecular weight: 200 kDa | |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | pkg of 100 μg |
| shipped in | wet ice |
| species reactivity | human (Tested), mouse (Predicted), rat (Predicted), African green monkey (Tested) |
| storage condition | avoid repeated freeze/thaw cycles |
| protect from light | |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:100 |
| western blot: 1:1000 | |
| UniProt accession no. | Q9H254   |
| Application: | Western blotting Dilution 1:1000 Tissue/Cell Type Used: monkey COS-Beta-4-Spectrin-His Immunofluorescence Dilution 1:100 Tissue/Cell Type Used: SK-N-BE |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Immunogen Description: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin |
| Immunogen: | Immunogen Region: C-terminal |
| Immunogen: | Immunogen Species: Human |
| Immunogen: | Immunogen type: Fusion protein |
| Other Notes: | Additional Product Details: Does not cross react with other Beta-spectrins Lot Expiration/Retest Date: Expires 1 year from purchase |
| Other Notes: | Target/Gene Description: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart . Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier . Purification method: Protein G purified Product type: Primary antibody Antibody structure: Whole Antibody Protein ID: NP_066022.2 |
| Physical form: | 50% glycerol, 0.1% sodium azide |
| Physical form: | PBS pH 7.4 |
| Preparation Note: | Derived from animal serum |
| Storage and Stability: | Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |