Monoclonal Anti-NaVbeta3-Allophycocyanin antibody produced in mouse
SIGMA/SAB5202176 - clone S396-29
Product Type: Chemical
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 3 Monoclonal Antibody, Clone S396-29. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min RT, 5min RT. Localization: Cell Membrane | Nucleus. Magnification: 60X.
Western Blotting Western Blot of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Nav Beta 3 Antibody, clone S396-29. Lane 1: MW Ladder. Lane 2: Mouse Brain (10 μg). Block: Unknown. Primary Antibody: Mouse Anti-Nav Beta 3, clone S396-29 at 1:1000 for O/N at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: Unknown. Predicted/Observed Size: ~40 kDa.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S396-29, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | Allophycocyanin conjugate |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | 40 kDa |
| NCBI accession no. | NP_620797.1   |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:100 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Fusion protein amino acids 1-215 (full-length) of rat NavBeta3 |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |