Monoclonal Anti-NPAS4-Atto 594 antibody produced in mouse
SIGMA/SAB5202260 - clone S408-79
Product Type: Chemical
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
Western Blotting Western Blot of Rat Brain showing detection of ~90 kDa NPAS4 protein using NPAS4 Antibody, clone S408-79. Lane 1: MW Ladder. Lane 2: Rat Brain (20 μg). Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Mouse Anti-NPAS4, clone S408-79 at 1:1000 for O/N at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S408-79, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | Atto 594 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 90 kDa |
| NCBI accession no. | NP_705890.1   |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:100 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Fusion protein amino acids 597-802 (C-terminus) of rat Npas4. |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |