Monoclonal Anti-Dibromo-tyrosine-Horseradish Peroxidase antibody produced in mouse
SIGMA/SAB5202413 - clone 9F12
Product Type: Chemical
Western Blotting Western Blot analysis of HeLa cells showing detection of 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Western Blotting Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 μM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin F-Actin stain (E,F) Dibromo-tyrosine Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9F12, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50 |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |