Monoclonal Anti-4-Hydroxy-2-hexenal-Allophycocyanin antibody produced in mouse
SIGMA/SAB5202464 - clone 6F10
Product Type: Chemical
Western Blotting Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal-BSA using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 μg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 μg). Lane 4: 4-hydroxy nonenal-BSA (2.0 μg). Lane 5: 4-hydroxy-2-hexenal (0.5 μg). Lane 6: 4-hydroxy-2-hexenal (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Flow cytometry Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: SH-SY5Y. Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 μM H2O2 for 24 hours.
Immunocytochemistry Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 μM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin F-Actin stain (E,F) 4-Hydroxy-2-hexenal Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6F10, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | Allophycocyanin conjugate |
| form | buffered aqueous solution |
| isotype | IgG2b |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | flow cytometry: 1:50 |
| immunofluorescence: 1:50 | |
| western blot: 1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH). |
| Physical form: | PBS pH 7.4, 50% glyercol, 0.09% Sodium Azide |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |