Anti-SMA antibody, Rabbit monoclonal
SIGMA/SAB5500002 - recombinant, expressed in proprietary host, clone SP171, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry Anti-SMA: Cat. No. SAB5500002: Immunohistochemistry of Actin-Smooth Muscle (SMA) in Human colon tissue with Actin-Smooth Muscle (SMA) antibody.
Immunoblotting Anti-SMA: Cat. No. SAB5500002: Immunoblotting analysis of Actin-Smooth Muscle (SMA) in HeLa cell lysate with Actin-Smooth Muscle (SMA) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP171, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | rabbit, rat, bovine, chicken, mouse, pig |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:50 |
| immunohistochemistry: 1:200 | |
| UniProt accession no. | P62736  |
| Application: | Anti-SMA antibody, Rabbit monoclonal has been used in: • western blotting • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Smooth muscle actin-α (SMA)/ α2-smooth muscle actin (ACTA2) interacts with β-myosin heavy chain and facilitates vascular smooth muscle cell contraction. The encoded protein regulates c-MET (tyrosine-protein kinase Met) and focal adhesion kinase (FAK) expression in human lung adenocarcinoma cells, which positively and selectively mediates tumor progression. Thus, SMA can be used as a potential prognostic biomarker and/or target for treating metastatic lung adenocarcinoma. Mutation in the gene is associated with the development of patent ductus arteriosus (PDA), bicuspid aortic valve (BAV), iris flocculi, livedo reticularis, cerebrovascular accident (CVA) and stenosis of the aortic vasa vasorum. In addition, variation in the gene expression leads to thoracic aortic aneurysms and dissections (TAAD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Smooth muscle actin-α (SMA), also known as α2-smooth muscle actin (ACTA2), is a cytoskeleton protein in smooth muscle cells. It is encoded by the gene mapped to human chromosome 10q23.31. SMA is a vascular smooth muscle specific isoform, |
| General description: | Smooth muscle actin-alpha (SMA) is a cytoskeleton protein in smooth muscle cells and their derived tumors such as leiomyoma and leiomyosarcoma. It is also expressed in myoepithelial cells of the breast and salivary gland, but not in fibroblasts, striated muscle, and myocardium. |
| Immunogen: | Synthetic peptide near the N-terminus of human SMA protein. |
| Physical form: | 0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |