Anti-N-Cadherin, Rabbit monoclonal
SIGMA/SAB5500023 - recombinant, expressed in proprietary host, clone SP90, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry Anti-N-Cadherin: Cat. No. SAB5500023: Immunohistochemistry of Cadherin-N/N-Cadherin in Human mesothelioma tissue with Cadherin-N/N-Cadherin antibody.
Flow Cytometry Anti-N-Cadherin: Cat. No. SAB5500023: Flow cytometric analysis of Monoconal Rabbit Anti-Cadherin-N antibody in HeLa (green) compare to negative control of Rabbit IgG (blue).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | SP90, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | mouse, bovine, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:100 |
| immunohistochemistry: 1:100 | |
| UniProt accession no. | P19022  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | N-cadherin is a 140kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. The function of N-cadherin is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of β-catenin. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. |
| Immunogen: | Synthetic peptide corresponding to C-terminus of human cadherin-N protein. |
| Physical form: | 0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |