Anti-CD25 antibody, Rabbit monoclonal
SIGMA/SAB5500055 - clone SP176, recombinant, expressed in proprietary host, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry Anti-CD25: Cat. No. SAB5500055: Immunohistochemistry of CD25 in Human tonsil tissue with CD25 antibody.
Immunoblotting Anti-CD25: Cat. No. SAB5500055: Immunoblotting analysis of CD25 in MOLT-4 cell lysate with CD25 antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP176, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | rat, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100 |
| western blot: 1:200 | |
| UniProt accession no. | P01589  |
| Biochem/physiol Actions: | Interleukin-2 (IL-2) is a cytokine that is essential in modulating activation, proliferation, and survival of several T-cell subsets. IL2RA gene has been associated with susceptibility to multiple sclerosis (MS). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | IL2RA (interleukin 2 receptor subunit α) gene codes for interleukin-2 receptor α-chain CD25. CD25 is expressed on activated T-cells. The activated T-cells liberate this molecule in the form of a soluble variant (referred to as soluble IL-2 receptor; sIL-2R) from the cell membrane into the circulation. The CD25 gene is located on human chromosome 10p15. The gene spans a length of 48kb with eight exons. |
| General description: | Interleukin-2 receptor subunit alpha, also known as CD25, is a type I transmembrane protein and the alpha chain of the IL-2 receptor. CD25 and CD122 form a heterodimer to act as a high-affinity receptor for IL-2. CD25 is expressed on activated T cells, activated B cells, some thymocytes, myeloid precursors, oligodendrocytes, and most B cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. |
| Immunogen: | Synthetic peptide derived from the C-terminus of human CD25 protein. |
| Physical form: | 0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |