Anti-CD3 antibody, Rabbit monoclonal
SIGMA/SAB5500058 - recombinant, expressed in proprietary host, clone SP7, tissue culture supernatant
Synonym: Anti-CD3epsilon; Anti-IMD18; Anti-T3E; Anti-TCRE
Product Type: Product-on-demand
Immunohistochemistry Anti-CD3: Cat. No. SAB5500058: Immunohistochemistry of CD3 in Human tonsil tissue with CD3 antibody.
Immunoblotting Anti-CD3: Cat. No. SAB5500058: Immunoblotting analysis of CD3 in Jurkat cell lysate with CD3 antibody.
Flow Cytometry Anti-CD3: Cat. No. SAB5500058: Flow cytometric analysis of Monoconal Rabbit Anti-CD3 antibody in Jurkat (green) compare to negative control of Rabbit IgG (blue).
| antibody form | tissue culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP7, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | pig, rat, dog, bovine, rabbit, chicken |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:100 |
| immunoblotting: 1:25 | |
| immunohistochemistry: 1:150 | |
| UniProt accession no. | P07766  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues. |
| Immunogen: | Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein. |
| Physical form: | 0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |