Anti-CD43 antibody, Rabbit monoclonal
SIGMA/SAB5500067 - clone SP55, recombinant, expressed in proprietary host, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry Anti-CD43: Cat. No. SAB5500067: Immunohistochemistry of CD43 in Human tonsil tissue with CD43 antibody.
Immunoblotting Anti-CD43: Cat. No. SAB5500067: Immunoblotting analysis of CD43 in Jurkat cell lysate with CD43 antibody.
Flow Cytometry Anti-CD43: Cat. No. SAB5500067: Flow cytometric analysis of Monoconal Rabbit Anti-CD43 antibody in Jurkat (green) compare to negative control of Rabbit IgG (blue).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP55, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: 1:100 |
| immunoblotting: 1:25 | |
| immunohistochemistry: 1:200 | |
| UniProt accession no. | P16150  |
| Application: | Anti-CD43 antibody, Rabbit monoclonal has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Cluster of differentiation 43 (CD43) is known to regulate cell cycle and cell relocation in both lymphoid and non-lymphoid tumor cells. It serves as a biomarker in chronic lymphocytic leukemia and other hematologic cancer diagnosis. CD43 is associated with cell movement, cell differentiation, apoptosis and immune homeostasis. Glycosylation and sialylation of CD43 at extracellular C-terminus, enables its purpose as a ligand for selectins. CD43 promotes extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling, which is necessary for actin cytoskeleton modifications. CD43 is associated with autoimmune diseases, including systemic lupus erythematosus, diabetes, human immunodeficiency virus (HIV) infection and Wiskott-Aldrich syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. It plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. |
| General description: | The SPN (sialophorin) gene is mapped to human chromosome 16p11.2 and encodesa transmembrane sialoglycoprotein cluster of differentiation 43 (CD43). It is localized to the surface of leukocytes and is expressed in hematopoietic cells. CD43 protein structure possesses a 23-amino acid transmembrane bridge connecting the N- and C- terminal regions. Its cytoplasmic tail is highly conserved. |
| Immunogen: | Synthetic peptide derived from the human CD43. |
| Physical form: | 0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |